Instruction/ maintenance manual of the product FV300 Olympus
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CONFOCAL LASER SCANNING BIOLOGICAL MICROSCOPES FV300 FLUOVIEW.
1 FV300 CONFOCAL LASER SCANNING MICROSCO PERFORMANCE FOR THE PERSONAL USER The FV300 is the ideal choice of laser scanning microscopes for personal users.
2 OPE q e r t y u i o !0 !1 !2 w q Optical fiber for laser introduction w Beam collimator e Polarizer r Dichromatic mirror t Excitation dichromatic mirror y XY galvanometer mirror scanners u Pupil len.
Preset the conditions for image acquisition and loading Storage of Acquisition Settings enables immediate, one-touch recall of all the relevant experimental settings and conditions.
Image tool bar X-Y-Z scanning operations and time- lapse observations both produce multiple images, which can be displayed in sequence simply by clicking the sequential mode button. Channel selection and image zooming are also available on the same menu.
5 X -t Slant Line-t FreeLine-t Y -t Y-Z-t X-Y-t ZoomIn-t X-Z-t X-Y-t X-Y-Z-t ZoomIn-Z-t Patch clamp BX61WI fixed stage upright microscope+translation stage Image acquisition ROI designation Intensity versus time measurement Calcium sparks in isolated cardiac myocyte Dr.
6 The 440nm diode laser can be added for CFP/YFP FRET imaging. A 440nm diode laser is optionally available for CFP/YFP imaging. The 440nm laser line ideally excites CFP, with minimal disturbance to YFP, and is therefore suitable for CFP/YFP FRET experiments.
7 PAPP for FRAP Application PAPP: Programmable Acquisition Protocol Processor Easy, reliable flow of experiments for fluorescence recovery after photobleaching.
8 Multi-point time lapse system High-magnification multi-point time lapse observation of living cells. Wide-ranging specimen observations for improved experiment throughput Use of a motorized XY stage allows the analysis of time lapse changes in many points scattered over a wide area.
9 Tile display Topographic projection Height of 3D structure indicated by color. X-Z Y-Z X-Y-Z X -Z Y -Z Using multiple 3D images to obtain accurate 3D structure analysis.
10 Link with patch clamping data • With PCs linked through a LAN, Physiolink can synchronize electrophysiological and confocal image data simultaneously. • With the same time stamp recorded in the two PCs, it is possible to access and analyze an image and its patch clamping data corresponding to the Physiolink software time scale.
11 Lucifer Yellow: retina ganglion cell TexasRed: dopamine-operated amacrine cell Prof. Shigetada Nakanishi Dept. of Biological Sciences, Kyoto Univ. Faculty of Medicine Applications Gallery Morpholog.
12 Fluorescent Proteins C elegans expressing beta-integrin fused to GFP Dr. Xioping Xhu and Dr. John Plenefisch University of Toledo, Dept. of Biology GFP-labeling of Drosophila adults Expression of DsRed in a zebrafish embryo Extended focus image of 5µmx30 slice Pr.
Fluorescence Dyes and Filters 300 400 500 600 700 BA530RIF BA550RIF BA650RIF BA430-460 BA465-495 BA480-495 BA470-520 BA510-540 BA520-550 BA505-550 BA505-525 BA480-510 BA535-565 BA540-590 BA560-600 BA5.
Objectives for fixed stage upright microscopes (using WI-UCD, WI-DICTHRA) 14 Item Specifications Laser light Visible light laser source Select from the following laser, to mounted on laser combiner so.
● All brands are trademarks or registered trademarks of their respective owners. ● Monitor images are simulated. Specifications are subject to change without any obligation on the part of the manufacturer. This catalog is printed by enviromentally-friendly waterless printing system with soy ink.
An important point after buying a device Olympus FV300 (or even before the purchase) is to read its user manual. We should do this for several simple reasons:
If you have not bought Olympus FV300 yet, this is a good time to familiarize yourself with the basic data on the product. First of all view first pages of the manual, you can find above. You should find there the most important technical data Olympus FV300 - thus you can check whether the hardware meets your expectations. When delving into next pages of the user manual, Olympus FV300 you will learn all the available features of the product, as well as information on its operation. The information that you get Olympus FV300 will certainly help you make a decision on the purchase.
If you already are a holder of Olympus FV300, but have not read the manual yet, you should do it for the reasons described above. You will learn then if you properly used the available features, and whether you have not made any mistakes, which can shorten the lifetime Olympus FV300.
However, one of the most important roles played by the user manual is to help in solving problems with Olympus FV300. Almost always you will find there Troubleshooting, which are the most frequently occurring failures and malfunctions of the device Olympus FV300 along with tips on how to solve them. Even if you fail to solve the problem, the manual will show you a further procedure – contact to the customer service center or the nearest service center
