Instruction/ maintenance manual of the product SP2600 Leica
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Leica SP2600 Ultramilling attachment Supplementary instruction manual Leica SP2600 To be used only in conjunction with instruction manual Leica SM2500 . V1.2 English 07/2002 Always keep this manual near the instrument. Read carefully prior to operating the instrument.
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3 Leica SP2600 Ultramilling attachment The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state- of-the-art technology as we understand it follow- ing thorough investigation in this field.
4 Supplementary instruction manual V 1.2 07/2002 Table of contents 1. Important information ........................................................................................................ ........ 6 1.1 Symbols in the text and their meaning .
5 Leica SP 2600 Ultramilling attachment Important information 6. Troubleshooting .............................................................................................................. ......... 44 7. Cleaning, disinfection and maintenance .
6 Supplementary instruction manual V 1.2 07/2002 1. Important information 1.2 Designated use The Leica SP2600 is designated to prepare milled surfaces and thin slices of specimen materials such as plastics and metals as well as of organic materials (such as bones, teeth) in medicine, biol- ogy and industry.
7 Leica SP 2600 Ultramilling attachment 1. Important information 1.3 Information on this instruction manual Instrument type This supplementary instruction manual must be used together with the Lei.
8 Supplementary instruction manual V 1.2 07/2002 2. Safety 2.1 Safety regulations This instrument has been built and tested in accordance with the same di- rectives and standards as the Leica SM2500. For a complete list of the directives and standards applied please refer to chapter 2.
9 Leica SP 2600 Ultramilling attachment 2. Safety 2.2 Built-in safety devices The instrument is equipped with the following safety devices: Plexiglass protective cover The miller is equipped with a protective cover made of plexiglass ( 1 ). When the cover is moved to the upper limit position ( 1, Fig.
10 Supplementary instruction manual V 1.2 07/2002 Be sure to read and comply with the safety instructions, warnings and cau- tions in this chapter as well as in chapter 2 of the Leica SM2500 instruc- tion manual, even if you are already fa- miliar with the operation and use of other Leica products.
11 Leica SP2600 Ultramilling attachment Safety instructions - transport and installation Caution! Only the vacuum cleaner offered by Leica or other vacuum cleaner brands rated at 230 V/ 50 Hz and .
12 Supplementary instruction manual V 1.2 07/2002 Safety instructions - cleaning disinfection and maintenance Only authorized service technicians may access the internal components of the instrument for service and repair! Cleaning detergents appropriate for the ultramilling attachment.
13 Leica SP 2600 Ultramilling attachment 3. Technical Data Ultramilling attachment Milling spindle (special design) ......................................................................................... type T SAV 60 x 160 Motor, rotation speed selectable in 100-rpm steps .
14 Supplementary instruction manual V 1.2 07/2002 4. Installation 4.1 Overview - Leica SP2600 Ultramilling attachment / basic instrument Leica SM2500 - instrument parts / functions 1. Leica SM2500 - basic instrument (sliding microtome) 1.1 Leica SP2600 - ultramilling attachment 1.
15 Leica SP2600 Ultramilling attachment 4. Installation 4.2 Leica SP2600 standard delivery Standard delivery includes: 1 Basic instrument Leica SP2600 (Ultramilling attachment) 1 Calibrating devic.
4.5 Installing the Leica SP2600 ultramilling attachment Place the carrier ( 1, Fig. 4.2 ) for the ultramilling attachment ( 3, Fig. 4.3 ) on the rear part of the Leica SM2500 microtome fitting the two pins of the carrier into the corresponding bores in the Leica SM2500 microtome.
4. Installation 4.6 Connecting the vacuum cleaner Milling dust is extracted by a vacuum cleaner, through a nozzle ( 1 ) located in the milling area. 230 V / 50 Hz Vacuum cleaners with a power draw of .
18 Supplementary instruction manual V 1.2 07/2002 4.7 Installing additional accessories Install further accessories as described in chapters 4.5.2 and 4.5.3, pages 18 and 19 of the Leica SM2500 instruction manual. Connect the foot switch. The foot switch must be connected - otherwise the instrument will not be operational.
19 Leica SP 2600 Ultramilling attachment 5.1 Overview of chapter 5 Operation Prior to working with the Leica SP2600 ultramilling attachment, be sure to read the following instruction manuals: The entire Leica SM2500 instruction manual except chapters 5.
20 Supplementary instruction manual V 1.2 07/2002 5.2 Adjusting the pre-millers in the calibrating device Preparatory steps Connect mains power supply ( 20 ) to miller cali- bration device ( 18 ). Connect mains power supply ( 20 ) to mains: the light bulb is switched on.
21 Leica SP2600 Ultramilling attachment Adjusting the miller The miller ( 3 ) is adjusted in the holder ( 2 ) along three different axis (x, y and z - see Fig. 5.3 , left). Adjustment along the x-axis is done outside the calibration device ( 18, Fig.
22 Supplementary instruction manual V 1.2 07/2002 Adjustment along the z-axis 1. Pull lever ( 12 ) at the left of calibration device ( 18 ) forward as far as it will go, thus moving contact plate ( 17 ) backwards to its rear limit position. This prevents the contact plate from hitting the milling edge during the adjustment procedure.
23 Leica SP2600 Ultramilling attachment 6. Insert the size-5 Allen key into regulating screw ( 4 ) insert size-2.5 Allen key into regu- lating screw ( 11 ) ( Fig. 5.7 ). The arrows in Fig. 5.7 indicate clock- wise sense of rotation. 7. Tighten/loosen screws ( 11 ) and ( 4 ) alternately to adjust miller ( 3 ) along the z-axis.
24 Supplementary instruction manual V 1.2 07/2002 Adjustment along the y-axis 1. Select reference value 1. The reference value will be needed later on to adjust the finishing miller to the same milling level as the pre- miller. Any reference value can be selected.
25 Leica SP2600 Ultramilling attachment 5.3 Adjusting the finishing millers in the calibrating device Carry out preparatory steps as per chapter 5.
26 Supplementary instruction manual V 1.2 07/2002 Adjusting the finishing miller Just like the pre-miller, the finishing miller is ad- justed in the miller holder along three different axis (see page 21). Adjustment along the x-axis As described for the pre-miller - see page 21.
27 Leica SP2600 Ultramilling attachment Fig. 5.12 4 11 3 7 6 Insert size-5 Allen key into regulating screw ( 4 ) insert size-2.5 Allen key into regulating screw ( 11 ) (Fig. 5.12 ) . The arrows in Fig. 5.12 indicate clock- wise sense of rotation. 7 Tighten/loosen screws ( 11 ) and ( 4 ) alternately to adjust miller ( 3 ) along the z-axis.
28 Supplementary instruction manual V 1.2 07/2002 Adjustment along the y-axis 1 Select the same reference value as was set for the pre-miller. Pre-miller and finishing miller used for milling a specific sample must be ad- justed using the same reference value.
29 Leica SP 2600 Ultramilling attachment 5.4 Specimen holder systems - how to combine individual components 5. Operation Configurations: 1. Base plate with dovetail guide (0383 09422) and holder for round specimens (0383 08992) = order no. 0383 08993 2.
30 Supplementary instruction manual V 1.2 07/2002 5.5 Clamping specimen For a detailed overview of the clamping systems available, please refer to chapter, 5.4, page 29. 5.5.1 Installing the base plates in the basic instrument For detailed instructions on how to install the base plates in the basic instrument, please refer to chapters 5.
31 Leica SP2600 Ultramilling attachment 5.5.3 Clamping the vacuum stages on/in the base plates Clamping the vacuum stages in the base plate with vise (Fig. 5.15) Fasten the vacuum stage on the vacuum stage adapter for the base plate with vise (with 4 Allen screws ( 1 ) / Allen key size 4).
32 Supplementary instruction manual V 1.2 07/2002 5. Operation 5.5.4 Connecting the vacuum pump / placing specimens onto the vacuum stages IMPORTANT: Prior to first use, every vacuum table surface must be milled once, just like a specimen surface.
33 Leica SP2600 Ultramilling attachment Placing specimens onto vacuum stage for 30 - 50-mm lids Fasten a lid suited to the size of the specimen to be milled onto the vacuum stage (slotted screws are part of standard delivery of vacuum stage lids - use slotted screwdriver).
34 Supplementary instruction manual V 1.2 07/2002 5.6 Preparatory steps prior to the milling procedure For a detailed description of all control elements located on the control unit, please refer to chapters 5.3 (Overview - control panel) and 5.
35 Leica SP 2600 Ultramilling attachment Insert the specimen (see chapters 5.5.2 and 5.5.4, pages 30 and 33 of this manual) or - if you need to mill a vacuum table prior to first use - see chapter 5.
36 Supplementary instruction manual V 1.2 07/2002 5.7 Pre-milling Select automatic mode of operation AUTO (button ( 5 ) - chapter 5.4.3, page 26 Leica SM 2500 instruction manual). In AUTO mode, set the milling parameters for the pre-milling procedure: Select STOP or PHOTO position (buttons ( 23 ) or ( 26 ) chapter 5.
37 Leica SP 2600 Ultramilling attachment Recommended milling thickness settings for pre-milling 10 µm - keep milling until surface is completely level. 5 µm - three entire sledge strokes (milling and return stroke). 3 µm - three entire sledge strokes (milling and return stroke).
38 Supplementary instruction manual V 1.2 07/2002 5.7.1 Perspective view of the pre-milling procedure 5. Operation Pre-milling procedure The milling spindle carrying the pre-miller rotates clockwise (circular arrow ( 1 )) while the specimen sledge passes underneath the miller in the direction of arrow ( 2 ).
39 Leica SP 2600 Ultramilling attachment 5. Operation Ending the pre-milling procedure Press RUN STOP or RUN ENABLE to stop the sledge (buttons ( 29 ), ( 30 ) - Chapter 5.4.10, page 33 - Leica SM2500 manual) - or, when working in PROG mode of operation, wait for the programmed number of strokes to be finished (see chapter 5.
40 Supplementary instruction manual V 1.2 07/2002 5.8 Finishing milling Attach the miller holder containing the finishing miller to the milling spindle (Allen screws / Allen key size 5) - see Fig.
41 Leica SP 2600 Ultramilling attachment Recommended sledge speed settings for finishing milling Milling stroke: 0.5 mm/s to 1.0 mm/s Return stroke: any speed Recommended rotational speed of miller for finishing milling < 2.000 rpm Recommended mode of operation for finishing milling Select continuous mode of operation.
42 Supplementary instruction manual V 1.2 07/2002 5.8.1 Perspective view of the finishing milling procedure 5. Operation Finishing milling procedure The milling spindle carrying the finishing miller rotates clockwise (circular arrow ( 1 )) while the specimen sledge passes underneath the miller in the di- rection of arrow ( 2 ).
43 Leica SP 2600 Ultramilling attachment Ending the finishing milling procedure Press RUN STOP or RUN ENABLE to stop the sledge (buttons ( 29 ), ( 30 ) - chapter 5.4.10, page 33 - Leica SM2500 manual) - or, when working in PROG mode of operation, wait for the programmed number of strokes to be finished (see chapter 5.
44 Supplementary instruction manual V 1.2 07/2002 6. Troubleshooting 6. Troubleshooting For all instructions on error detection and trouble shooting, please refer to chapter 6, page 64 of the Leica SM2 500 instruction manual .
45 Leica SP 2600 Ultramilling attachment 7. Cleaning, disinfection and maintenance 7.1 Cleaning and disinfection Only authorized service technicians may access the internal com- ponents of the ins.
46 Supplementary instruction manual V 1.2 07/2002 7. Cleaning, disinfection and maintenance 7.2 Maintenance Only authorized service technicians may access the internal com- ponents of the instrume.
47 Leica SP 2600 Ultramilling attachment 7.2.2 Replacing the light bulb in the calibration device Remove base cover ( 2 ) of calibration device ( 1 ) (unscrew 4 slotted screws ( 3 )). Loosen light bulb holder ( 5 ) (metal bridge with lamp socket) (unscrew 2 slotted screws ( 4 )).
48 Supplementary instruction manual V 1.2 07/2002 8. Overview - accessories 8.1 Miller holders and millers Miller holder Holder for pre- and finishing miller Pre-miller Pre-miller D - with dia.
49 Leica SP 2600 Ultramilling attachment 8.5 Vacuum stages and matching lids / perforated specimen holders Vacuum stage for Leica SP2600 - basic body for lids 30 - 50 mm Lid, 30 mm, for Le.
50 Supplementary instruction manual V 1.2 07/2002 8.7 Additional accessories Vacuum cleaner and vacuum cleaner accessories Vacuum cleaner Fakir S20, with connection hose 230 V / 50 Hz on.
51 Leica SP 2600 Ultramilling attachment 9. Warranty and service For all information on warranty and service, please refer to chapter 9, pages 74 - 76 of the Leica SM2500 instruction manual.
An important point after buying a device Leica SP2600 (or even before the purchase) is to read its user manual. We should do this for several simple reasons:
If you have not bought Leica SP2600 yet, this is a good time to familiarize yourself with the basic data on the product. First of all view first pages of the manual, you can find above. You should find there the most important technical data Leica SP2600 - thus you can check whether the hardware meets your expectations. When delving into next pages of the user manual, Leica SP2600 you will learn all the available features of the product, as well as information on its operation. The information that you get Leica SP2600 will certainly help you make a decision on the purchase.
If you already are a holder of Leica SP2600, but have not read the manual yet, you should do it for the reasons described above. You will learn then if you properly used the available features, and whether you have not made any mistakes, which can shorten the lifetime Leica SP2600.
However, one of the most important roles played by the user manual is to help in solving problems with Leica SP2600. Almost always you will find there Troubleshooting, which are the most frequently occurring failures and malfunctions of the device Leica SP2600 along with tips on how to solve them. Even if you fail to solve the problem, the manual will show you a further procedure – contact to the customer service center or the nearest service center
