Instruction/ maintenance manual of the product 71-5000-15 AD GE
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Instructions 71-5000-15 AD Pre-activated media GE Healthcare CNBr -activated Sepharose 4 Fast Flow Introduction Th e pre pa ra ti on an d us e of a f f ini ty c hro ma to gra phy m ed ia b y cou pl in g bio sp ec if i c li gan ds t o CNBr-a c ti vat ed m at ri ce s is a w ide ly u se d, su cce ss f ul an d we ll -d oc ume nte d technique.
p. 2 1. Product description CNB r -a c ti vat ed S ep har os e 4 Fas t Fl ow i s a be ad -fo rm ed , hig hl y cro ss- lin ke d pre - ac t iva te d ma tr ix p rod uce d by r ea ct in g Se ph aro se 4 Fa s t Fl ow wi th cy an og en b ro mid e ( CNB r) .
p. 3 Ta b l e 1 . Medium charac teristic s. Me an p ar t ic le si ze 90 μ m Part icle siz e range 45– 1 65 μ m Be ad s t ru c ture Hi gh ly cr os s-l in ked 4 % ag aro se, s ph er ic al Linear flowrate Ba se m at r ix 150 –250 c m /h , 0.
p. 4 In or de r to ret ain m ax im um b ind in g cap ac ity o f CNBr-a c ti vat ed S ep har os e 4 Fas t Fl ow p ri or t o cou pl ing t he l ig an d, us e co ld ( 0 – 4 ° C) s olu t ion s .
p. 5 5. Was h awa y ex es s li gan d wi t h at l ea st 5 m ed ium v ol ume s of co up li ng b uf fe r. 6. Af te r cou pl in g, no n- re ac te d gro up s on t he m edi um s ho uld b e b lo cke d. T ran s fer t he m edi um to 0 .1 M T ris -H Cl bu f fe r pH 8.
p. 6 Lar ge sc al e col umns • BP G TM var ia bl e be d, g la ss co lu mn s. In ne r di ame te rs f rom 10 0 –450 m m, be d vol um es f rom 2.4 – 131 lit res ; be d he ig ht ma x 83 cm . • CHRO MAFLO W TM var ia bl e be d col um ns . Inn er d iam et er s fro m 40 0 – 60 0 mm .
p. 7 7 . Wh en t he b ed h as s tab il ize d, c lo se t he b ot tom o ut le t an d st op t he p ump . 8. If u sin g a pa cki ng r es er vo ir, dis con ne ct t he r es er vo ir a nd f i t th e ad apt or to th e co lum n.
p. 8 Th e cal cul ate d pl at e numb er w ill v ary d ep end ing o n th e tes t co nd iti ons and i t sho uld t he ref or e be us ed a s a ref er enc e val ue on ly. I t is a ls o imp o r tan t th at co nd it io ns a nd e qui pm en t are ke pt co ns ta nt so t ha t re sul t s are co mp ar abl e.
p. 9 Th e red uce d pl at e he igh t is ca lcu la te d : HET P/d wh ere d i s th e dia me ter o f th e be ad . As a g ui de lin e, a va lue o f <3 is n or ma ll y acceptable . Th e pe ak s hou ld b e sy mme tr ic al, a nd t he a sym me tr y fa ct or a s clo se a s po ss ibl e to 1 ( va lue s b et we en 0.
p. 10 5. Cleaning, Sanitization and Storage For b es t p er fo r man ce of co up le d CNB r -a c ti va ted S ep ha ros e 4 Fas t Fl ow o ver a lo ng wo rk in g li fe, fol lo w th e ge ner al pr oce du res d es cr ib ed b el ow. In al l cas es , we re co mme nd t es ti ng t he p ro ced ure s at s ma ll s cal e f ir st .
p. 11 or Was h th e col umn w it h 3– c ol umn v ol ume s of 70 % et han ol . Was h im me di ate ly w it h at l ea s t 5 col umn vol um es of s te ri le f i lte re d bin di ng b uf f er. Sanitization San it iz at io n in ac ti va tes m ic rob ia l cont am ina nt s in t he p ac ked c olu mn a nd rel at ed e qui pm ent .
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An important point after buying a device GE 71-5000-15 AD (or even before the purchase) is to read its user manual. We should do this for several simple reasons:
If you have not bought GE 71-5000-15 AD yet, this is a good time to familiarize yourself with the basic data on the product. First of all view first pages of the manual, you can find above. You should find there the most important technical data GE 71-5000-15 AD - thus you can check whether the hardware meets your expectations. When delving into next pages of the user manual, GE 71-5000-15 AD you will learn all the available features of the product, as well as information on its operation. The information that you get GE 71-5000-15 AD will certainly help you make a decision on the purchase.
If you already are a holder of GE 71-5000-15 AD, but have not read the manual yet, you should do it for the reasons described above. You will learn then if you properly used the available features, and whether you have not made any mistakes, which can shorten the lifetime GE 71-5000-15 AD.
However, one of the most important roles played by the user manual is to help in solving problems with GE 71-5000-15 AD. Almost always you will find there Troubleshooting, which are the most frequently occurring failures and malfunctions of the device GE 71-5000-15 AD along with tips on how to solve them. Even if you fail to solve the problem, the manual will show you a further procedure – contact to the customer service center or the nearest service center
