Instruction/ maintenance manual of the product 4411-0039-CE Princeton
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4411-0039-CE Version 6.C April 18, 2006 * 4411-0039-CE*.
© Copy right 2003-2006 division of Roper Scientific, Inc. Princeton Instruments, a 3660 Quakerbridge Rd Trenton, NJ 08619 TEL: 800-874-9789 / 609-587-9797 FAX: 609-587-1970 All rights reserved. No part of this publication may be reproduced by any means without the written permission of Princeton Instruments, a division of Roper Scientific, Inc.
Table of Contents Chapter 1 In troduction ........................................................................................ 9 Introduction .........................................................................................................
iv MicroMAX System User Manual Version 6.C Chapter 6 Advan ced Topi cs ............................................................................. 73 Introduction ......................................................................................
Table of Contents v Programmable Interface .................................................................................................. 132 A/D Converter............................................................................................
vi MicroMAX System User Manual Version 6.C Figures Figure 1. MicroMAX Cameras and Controller .................................................................. 9 Figure 2. Power Switch Loca tion (ST-133A and ST-133B) ..................................
Table of Contents vii Figure 49. Free Run Mode Timing Diagram ................................................................... 95 Figure 50. Setup using to Trigger an Event ....................................................... 95 Figure 51. Timing for Experiment Setup shown in Figure 50 .
viii MicroMAX System User Manual Version 6.C Table 7. Approximate Readout Time for the Fram e-Transfer CCD Array ...................... 63 Table 8. Approximate Readout Time for the Interline CCD Arrays................................ 67 Table 9. Readout Rates for PI 1300 × 1030 Array at 1 MHz .
Chapter 1 Introduction Introduction The Princeton Instruments MicroMAX system is a high-speed, low-noise CCD camera system designed for demanding imaging applications and is an optim al system for use in fluorescence microscopy applications such as high-resolution immunofluorescence, FISH or GFP imaging.
10 MicroMAX System User Manual Version 6.C Camera Introduction: The function of the camera is to collect very low intensity light and convert the energy into a quantitative, electronic signal (photo-electrons) over a two dimensional space.
Chapter 1 Introduction 11 The standard MicroMAX Controller enables both high-speed and high-precision readout capabilities. It can collect 16-bit images at a readout rate of up to 1 million pixels per second (1 MHz) in the high-speed mode or at 100 thousand pixels per second (100 kHz) in the optional precision mode (16-bit).
12 MicroMAX System User Manual Version 6.C About this Manual Manual Organization This manual provides the user with all the information needed to install a MicroMAX camera and place it in operation. Topics cove red include a detailed description of the camera, installation, cleaning, specifications and more.
Chapter 1 Introduction 13 Appendix E , USB 2.0 Limitations covers the currently known limitations associated with operating under the USB 2.0 interface. Declarations of Conformity contains the Declaration of Conform ity for 1 MHz (includes 100 kHz/1MHz) MicroMAX systems.
14 MicroMAX System User Manual Version 6.C Precautions To prevent permanently damaging the syst em, please observe the following precautions: • Always switch off and unplug the ST-133 Cont roller before changing your system configuration in any way.
Chapter 2 System Component Descriptions MicroMAX Camera CCD Array: MicroMAX offers a choice of CCD technologies to improve quantum efficiency (QE) and blue/green sensitivity.
16 MicroMAX System User Manual Version 6.C forced air. CCD temperature is controlled and m onitored by via the host computer and the ST-133 Controller.
Chapter 2 System Component Descriptions 17 The fan is designed for low-vibration and does not adversely affect the image. For the fan to function properly, free circulation must be maintained between the rear of the camera and the laboratory atmosphere.
18 MicroMAX System User Manual Version 6.C ST-133 Co n tr ol le r Electronics: Th e M od el ST-133 is a co mpa ct , h ig h pe rf or man ce CC D C ame ra Controller for operation with Princeton Instruments cameras.
Chapter 2 System Component Descriptions 19 Always turn the power off at the Controller before connecting or disconnecting any cable that interconnects the camera and controller or serious damage to the CCD m ay result. This damage is NOT covered by the manufacturer’s warranty.
20 MicroMAX System User Manual Version 6.C The descriptions of the rear panel connectors are keyed to the accompanying figure. Depending on your system, either the TAXI or the USB 2.0 Interface Control Module will be installed in the second from the left slot (as you face the rear of the ST-133).
Chapter 2 System Component Descriptions 21 # Feature 10. Serial COM Connector: Provides two-way serial communi cation between the controller and th e ho s t c om pu t er .
22 MicroMAX System User Manual Version 6.C Selecting the wrong shutter setting will result in improper functioning of the shutter and may cause premature shutter failure. WARNING Shutter t exp t c t R Shutter T ype Compensation Time NONE 200 nsec Electronic 6.
Chapter 2 System Component Descriptions 23 Cables Detector-Controller: 1 MHz or 100kHz/1MHz systems. The standard 10' cable (6050-0321) has DB-25 Male connectors with slide-latch locking hardware.
24 MicroMAX System User Manual Version 6.C PVCAM is the standard software interface for cooled CCD cameras from Roper Scientific. It is a library of functions that can be used to control and acquire data from the camera when a custom application is being written.
Chapter 3 Installation Overview The list and diagrams below briefly descr ibe the sequence of actions required to hookup your system and prepare to gather data. Refer to the indicated references for more detailed information. This list assum es that the application software is Princeton Instruments WinView/32.
26 MicroMAX System User Manual Version 6.C Action Reference 13. Turn on the computer and begin running th e WinX application. WinView/32 manual 14. Run the Camera Detection wizard or load the defaults from the controller. Chapter 5 Operation, page 40 , 48 , or 53 WinView/32 or WinSpec/32 m anual 15.
Chapter 4 System Setup Unpacking the System During the unpacking, check the system co mponents for possible signs of shipping damage. If there are any, notify Princeton Instruments and file a claim with the carrier. If damage is not apparent but camera or c ontroller specifications cannot be achieved, internal damage may have occurred in shipm ent.
28 MicroMAX System User Manual Version 6.C System Requirements Power Detector: The MicroMAX detector receives its power from the controller, which in turn plugs into a source of AC power. ST-133: The ST-133 Controller can operate from any one of four different nominal line voltages: 100, 120, 220, or 240 V AC.
Chapter 4 System Setup 29 USB 2.0 Protocol: • AT-com patible computer with Pentium 3 or better processor and runs at 1 GHz or better. • Windows 2000 (with Service Pack 4), Windows XP (with Service Pack 1) or later operating sy stem. • Native USB 2.
30 MicroMAX System User Manual Version 6.C Installing the Application Software Installation is performed via the WinView/32 installation process. If you are installing WinView or WinSpec for the first time, you should run the installation before installing the Princeton Instruments (RSPI) PCI or USB2.
Chapter 4 System Setup 31 To Install a PCI Serial Buffer Card in the Host Computer: 1. Review the docum entation for your com puter and PCI card before continuing with this installation. 2. To avoid risk of dangerous electrical s hock and dam age to the com puter, verify that the computer power is OFF.
32 MicroMAX System User Manual Version 6.C Setting up a USB 2.0 Interface Administrator privileges a re required under Windows NT ® , Windows ® 2000, and Windows ® XP to install software and hardware. Your system has been configured to use the USB communication protocol if the Interface Control Module installed in the ST-133 has a USB 2.
Chapter 4 System Setup 33 7. On the Driver tab, click on the Update Driver… button. You may have to wait a minute or so before you will be allowed to click on the button. 8. When the Upgrade Device Driver wizard appears, click on Next . Select the Search for a suitable driver … radio button.
34 MicroMAX System User Manual Version 6.C Windows Version USB INF Filename Located in "Windows"/IN F directory* USB Properties DLL Located in "Windows"/System3 2 directory USB Device Driver Name Located in "Windows"/System32/Driver s directory Windows ® 2000 and XP rsusb2k.
Chapter 4 System Setup 35 Note: C-mount cameras are shipped with a dust c over lens installed (identifiable by its red rim). Although this lens is capable of providing im ages, its throughput is low and the image quality is not as good as can be obtained with a high quality camera lens.
36 MicroMAX System User Manual Version 6.C allow the user to reduce exposure times, increasing temporal resolution and reducing photodamage to the living specime n. Another method to minim ize photodamage to bi ological preparations is to synchronize a shutter on the excitation pathway to the e xposure period of the camera.
Chapter 4 System Setup 37 the appropriate setscrews on the microscope to secure th ut port of the microscope. e bottom clamp to the outp Microscope Type Diagnostic Instruments Bottom Clamp Type Leica .
38 MicroMAX System User Manual Version 6.C 1X HRP 100-NIK "L" bottom clamp Figure 9. Bottom Clamp secured to Relay Lens Microscope optics have very high transmission efficiencies in the infrared region of the spectrum.
Chapter 4 System Setup 39 Selecting the Shutter Setting The Shutter Setting push switch on the rear of the Controller sets the shutter hold voltage. Each shutter type, internal or external, re quires a different setting. Consult the table below for the proper setting for your shutter.
40 MicroMAX System User Manual Version 6.C USB 2.0 Cable (6050-0494) Turn the Controller power OFF (OFF = 0, ON = |) and the Computer power OFF before connecting or disconnecting the Controller-Computer (TAXI) cable. Caution To Connect the USB 2.0 Cable: 1.
Chapter 4 System Setup 41 Figure 11. Camera Detection Wizard - Wel come dialog box 4. Follow the instructions on the dialog boxes to perform the initial hardware setup: this wizard enters default parameters on the Hardware Setup dialog box tab pages and gives you an opportunity to acquire a test image to confirm the system is working.
42 MicroMAX System User Manual Version 6.C 5. When the PVCAM dialog box ( Figure 13 ) is displayed, click in the Yes radio button, click on Next and continue through the wizard. After the wizard is finished, the Controller/Camera tab card will be displayed with the Use PVCAM checkbox selected.
Chapter 5 Operation Introduction Once the MicroMAX camera has been inst alled, camera operation is basically straightforward. In most applications you simply establish optimum performance using the Fo.
44 MicroMAX System User Manual Version 6.C • In Acquire mode, every frame of data collected can be automatically stored (the completed dataset may include multiple frames with one or m ore accumulations). This mode would ordinarily be selected during actual data collection.
Chapter 5 Operation 45 Imaging Field of View When used for two-dimensional imaging applications, the MicroMAX cam era closely imitates a standard 35 mm camera. Since the CCD is not the same size as the film plane of a 35 mm cam era, the field of view at a given distance is somewhat different.
46 MicroMAX System User Manual Version 6.C In addition to intensity-scaling, you also need to be concerned about how the array pixels map to the video display.
Chapter 5 Operation 47 First Light (Imaging) The following paragraphs provide step-by-step instructions for placing your MicroMAX system in operation the first time. The intent of this simple procedure is to help you gain basic familiarity with the operation of your system and to show that it is functioning properly.
48 MicroMAX System User Manual Version 6.C Cabling If the system cables haven’t as yet been insta lled, make sure that the ST-133 and the host computer are turned off and then ma ke the cable connections as follows: See Figure 17.
Chapter 5 Operation 49 • Controller version: 4 or higher • Camera type: Select array installed in your camera. MicroMAX:512FT = EEV 512× 512 FT C C D 5 7 MicroMAX:512BFT = EEV 512× 512 FT C C D .
50 MicroMAX System User Manual Version 6.C Experiment Setup Timing tab page (Acquisition|Experiment Setup…): • Timing Mode: Free Run • Shutter Control: Norm al • Safe Mode vs.
Chapter 5 Operation 51 d. Set the focus adjustment of the lens fo r maxim um sharpness in the viewed image. e. hen divert the light to the camera and lower the o h nts relay lens without touching the main focusing o is normally o ugh e microscope to f.
52 MicroMAX System User Manual Version 6.C addition, you should have a basic understandi ng of how the system hardware is used. Other topics, which could be quite important in certain situations, are discussed in the following chapters. See the appropriate ap p lication software m anual for information on using the software to control the system.
Chapter 5 Operation 53 Setting the Parameters Note: The following procedure is based on WinSpec/32: you will need to modify it if you are using a different application. Basic familiarity with the WinSpec/32 software is assumed. If this is not the case, you may want to review the software manual or have it available while performing this procedure.
54 MicroMAX System User Manual Version 6.C malfunction. Once lock is established, the temperature will be stable to within ±0.05°C. Note: If you are using the USB 2.0 interface, the Detector Temperature dialog box will not display temperature information while you are acquiring data.
Chapter 5 Operation 55 4. Slowly move the detector in and out of focus. You should see the spectral line go from broad to narrow and back to broad. Leave the detector set for the narrowest achievable line.
56 MicroMAX System User Manual Version 6.C CCD Array Architecture Charge coupled devices can be roughly thought of as a two-dimensional grid of individual photodiodes (called pixels), each conn ected to its own charge storage “well.
Chapter 5 Operation 57 NOT SCAN Mechanical Shutter Acquire Readout Closed Open Exposure time Shutter compensation t exp t c Figure 19. CCD Exposure with Shut ter Compensation Note that NOT SCAN is low during rea dout, high during exposure, and high during shutter compensation time.
58 MicroMAX System User Manual Version 6.C Full-Frame For full-frame CCDs, the MicroMAX camera is us ually equipped with an integral shutter. If a full-frame MicroMAX is being operated w ithout a shutter, smearing can be avoided by ensuring that no light falls on the CCD during readout.
Chapter 5 Operation 59 on-screen indication allows easy verification of temperature lock. In addition, the TEMP LOCK indicator on the back of the controller lights GREEN to indicate that lock has been achieved (for more information, refer to the description of the ST-133 rear panel features, page 20 ).
60 MicroMAX System User Manual Version 6.C If you observe a sudden change in the baseline signal you may have excessive humidity in the vacuum enclosure of the camera. Turn off the controller and have the cam era repumped before resuming norm al operation.
Chapter 5 Operation 61 to the output amplifier where the electrons are grouped as electrons/count. This result leaves the CCD and goes to the preamp lifier where gain is applied. WinView and WinSpec allow you to specify the type of readout, binning, the output am plifier, and the gain (the number of electrons required to generate an ADU).
62 MicroMAX System User Manual Version 6.C After charge is shifted out of each pixel the remaining charge is zero, meaning that the array is immediately ready for the next exposure. Below are the equations that determine the ra te at which the CCD is read out.
Chapter 5 Operation 63 Frame Transfer The MicroMAX fully supports frame transfer r eadout. Operation in this mode is very similar to the operation of video rate cameras . Half of the CCD is exposed continuously, raising the exposure duty cycle to nearly 100%.
64 MicroMAX System User Manual Version 6.C Interline In this section, a simple 6 × 3 pixel interline CCD is used to demonstrate how charge is shifted and digitized. As described below, tw o different types of readout, overlapped and non-overlapped can occur.
Chapter 5 Operation 65 4 3 12 Empty Readout Register . Exposure has ended and image is being transf erred to storage cells. Image has been shifted to storage cells, first line has been shifted to Readout Register , and second exposure begins . Charge from first cell has been shifted to the Output Node.
66 MicroMAX System User Manual Version 6.C Part 4 of Figure 24 illustrates the situation at the end of the readout. Both the imaging and storage cells are empty. In Free Run operation, the imaging cells will be switched back on immediately, allowing charge accumulation to begin.
Chapter 5 Operation 67 The readout time is approximately given by: t R = [N x · N y · (t sr + t v )] + (N x · t i ) (2) where N x is the smaller dimension of the CCD N y is the larger dimension of the CCD.
68 MicroMAX System User Manual Version 6.C On-Chip Binning Binning is the process of adding the data from adjacent pixels together to form a single pixel (som etimes called a super-pixel), and it can be accom plished in either hardware or software.
Chapter 5 Operation 69 The readout rate for n × n binning is approximated using a m ore general version of the full resolution equation. The modified equation is: (3) On-Chip Binning for Interline Binning is the process of adding the data from adjacent cells together, and it can be accom plished in either hardware or software.
70 MicroMAX System User Manual Version 6.C Software Binning One limitation of hardware binning is that the shift register pixels and the output node are ty pically only 2-3 times the size of imaging pixels as shown in Table 10 .
Chapter 5 Operation 71 the Analog Gain to High so that the signal can be spread over as much of the A/D range as possible. Users who consistently measure low-level signals may wish to select High , which reduces some sources of noise. Users who measure high-level signals m ay wish to select Low to allow digitization of larger signals.
72 MicroMAX System User Manual Version 6.C Note: In WinView and WinSpec, the ADC rate can be changed on the Experiment Setup|ADC tab page..
Chapter 6 Advanced Topics Introduction Previous chapters have discussed setting up the hardware and the software for basic operation. This chapter discusses topics associated with experiment synchronization (set up on the Experiment Setup| Timing tab page in WinView), TTL control, and the Kinetics mode option.
74 MicroMAX System User Manual Version 6.C Standard Timing Modes Overview The Princeton Instruments ST-133 Controller h as been designed to allow the greatest possible flexibility when synchronizing data collection with an experiment.
Chapter 6 Advanced Topics 75 Shutter Open Close Open Close Open Close Read Read Read First exposure Data stored Third exposure Data stored t exp t c t R Second exposure Data stored NOT SCAN Figure 29. Free Run Timing Diagram External Sync In this mode all exposures are synchronized to an external source.
76 MicroMAX System User Manual Version 6.C Shutter opens Shutter remains open for preprogrammed exposure time System waits while shutter closes Shutter opens Controller waits for External Sync pulse Controller waits for External Sync pulse (shutter preopen) (shutter normal) Figure 30.
Chapter 6 Advanced Topics 77 External Sync with Continuous Cleans The third timing mode available with the MicroMAX cam era is called Continuous Cleans.
78 MicroMAX System User Manual Version 6.C Shutter (Normal) Open Close Open Close Open Close Read Read Read Shutter (Preopen) Open Close Open Close Open Close External Sync NOT SCAN Figure 33.
Chapter 6 Advanced Topics 79 another as rapidly as possible as shown in Figure 34 . In these figures, Shutter indicates the programmed exposure time. If a shutter were present and active, it would also be the actual exposure time. Prior to the first readout, clean cycles are performed on the array.
80 MicroMAX System User Manual Version 6.C External Sync (negative polarity shown) NOT SCAN Shutter t w1 t c t exp cleans acquisition t R t R t R t R actual exposure time Figure 36.
Chapter 6 Advanced Topics 81 where the cell contents can be contaminated by the charge in other cells as data is move across the CCD and under the mask. There are two timing options available in the overlapped mode, Free Run and External Sync. ( None should be the Shutter Type selecti on if using WinView/32 software and operating without a shutter.
82 MicroMAX System User Manual Version 6.C Shutter External Sync (negative polarity shown) t exp t w1 50ns min.pulse between frames t R t R t R t R actual exposure time cleans acquisition NOT SCAN Figure 37.
Chapter 6 Advanced Topics 83 Fast and Safe Speed Modes The WinSpec/32 Experiment Setup Timing tab page allows the user to choose Fast or Safe Mode . Figure 40 is a flowchart comparing the two modes. In Fas t Mode ope ration, the MicroMAX runs according to the tim ing of the experiment, with no interruptions from the computer.
84 MicroMAX System User Manual Version 6.C Backg round or flatfield on? Computer programs camera with e xposure and binning parameters Start acquisition command sent from computer to camera Cleans per.
Chapter 6 Advanced Topics 85 TTL Control Fully supported by WinView/WinSpec Version 2.5 when the communication protocol is TAXI (PCI), this feature is not su pported when the protocol is USB 2.0. Introduction This connector provides 8 TTL lines in, 8 TTL lines out and an input control line.
86 MicroMAX System User Manual Version 6.C Table 12 illustrates this coding for decimal values 0 through 7. Obviously this table could easily be extended to show the c oding for values all the way to 255.
Chapter 6 Advanced Topics 87 Pin # Assignment Pin # Assignment 1 IN 1 14 IN 2 2 IN 3 15 IN 4 3 IN 5 16 IN 6 4 IN 7 17 IN 8 5 GND 18 GND 6 EN/CLK 19 Reserved 7 (future use) 20 GND 8 GND 21 OUT 2 9 OUT 1 22 OUT 4 10 OUT 3 23 OUT 6 11 OUT 5 24 OUT 8 12 OUT 7 25 GND 13 Reserved Table 13.
88 MicroMAX System User Manual Version 6.C widely available and can often be obtained locally, such as at a nearby Radio Shack ® store. A list of possibly useful items follows.
Chapter 6 Advanced Topics 89 Kinetics Mode Kinetics operation requires that the Kinetics op tion has been installed. Additionally, WinView (or WinSpec), version 2.5.18.1 or higher, is required when operating under USB 2.0. Introduction Kinetics mode uses the CCD to expose and store a limited num ber of images in rapid succession.
90 MicroMAX System User Manual Version 6.C Kinetic Timing Modes Kinetics mode operates with three timing m odes: Free Run, Single Trigger, and Multiple Trigger.
Chapter 6 Advanced Topics 91 NOT SCAN Signal Shutter opening time Shutter closing time Readout ST ART A CQUIRE command from the software issent automatically when ACQ UIRE or FOCUS is click ed on in the software . Exposure Shift SHUTTER Signal ST ART A CQUIRE Figure 46.
92 MicroMAX System User Manual Version 6.C NOT SCAN Signal Shutter opening time Shutter closing time Readout ST ART A CQUIRE command from the software issent automatically when ACQ UIRE or FOCUS is click ed on in the software . External T riggers Exposure Shift SHUTTER Signal ST ART A CQUIRE Figure 48.
Chapter 7 MicroMAX DIF Camera (Double Image Feature) Supported by WinView/WinSpec Version 2.5 when the communication protocol is TAXI (PCI), this feature is not supported when the protocol is USB 2.0. Introduction This chapter describes operation of the Micr oMAX DIF system.
94 MicroMAX System User Manual Version 6.C ESABI: Allows two fast images of equal duration to be acquired. Unlike the IEC and EEC modes, in the ESABI mode, two pulses are applied to the Ext.
Chapter 7 MicroMAX DIF Camera 95 exposure. In Free Run operation, the time that remains low will ty the range of 400 to 600 ns. pically be in READ Y 400 ns the rising edge of the EXPOSURE Figure 49.
96 MicroMAX System User Manual Version 6.C Summary of Free Run Timing mode • Allows you to capture single images. • Requires that the switch, if present on the back of the camera, be set to INACTIVE . • Uses Exposure Time set via software Experiment Setup.
Chapter 7 MicroMAX DIF Camera 97 Images 5 μ s Mechanical Shutter 8 ms 8 ms EXT . SYNC. 200 ns NOTSCAN 5 μ s Image1 Image 2 ~200 ns Laser Output >200 ns Laser 1 Laser 2 READY Figure 52. Timing Diagram for Typical IEC Measurement Figure 53 illustrates the interconnections that might be used for such an experiment with two lasers.
98 MicroMAX System User Manual Version 6.C Example 2: As shown in Figure 55 , the signal from the controller can be used to trigger the controller by connecting it back into the EXT SYNC connector. At the same time, it can be used to trigger a DG-535.
Chapter 7 MicroMAX DIF Camera 99 first image exposure time. The controller signal goes low when the camera is ready to begin imaging. Figure 56 illustrates an EEC timing example. EXT . SYNC. (A) Images t exp Mechanical Shutter 8 ms 8 ms NOT SCAN Image 1 t sync Image 2 200 ns READY Figure 56.
100 MicroMAX System User Manual Version 6.C Image 1 EXT . SYNC. (A) Images t trig Mechanical Shutter 8 ms 8 ms NOT SCAN t trig Image 2 No Signal Integration t exp t exp 200 ns 200 ns READY Figure 57.
Chapter 7 MicroMAX DIF Camera 101 Background subtraction allows you to automati cally subtract any constant background in your signal. This includes both constant off sets caused by the amplifier system in the controller as well as time-dependent (but consta nt for a fixed integration time) buildup of dark charge.
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Chapter 8 Virtual Chip Mode Fully supported by WinView/WinSpec Version 2.5 when the communication protocol is TAXI (PCI), this feature is not supported when the protocol is USB 2.0. Introduction Virtual Chip mode (a WinView/32 option) is a special fast-acquisition technique that allows frame rates in excess of 100 fps to be obtained.
104 MicroMAX System User Manual Version 6.C 1 MHz 100 kHz ROI (msec/Frame) (FPS) (msec/Frame) (FPS) 164 x 164 29.8 33.6 287 3.5 96 x 96 10.9 91.7 102 9.8 84 x 84 8.65 115 79.8 12.5 64 x 64 5.26 190 47.6 21.0 56 x 56 4.22 237 37.4 36.7 47 x 47 3.08 324 26.
Chapter 8 Virtual Chip Mode 105 System Connection Diagram: Computer 110/220 Controller Detector EXPERIMENT 110/220 Camera Detector-Controller Serial Com or USB 2.0 Interface cable (T AXI or USB 2.0) Microscope Figure 59. System Diagram Procedure: 1. Verify that the power is OFF for ALL system components (including the host computer).
106 MicroMAX System User Manual Version 6.C Interface tab card • Type: the appropriate interface card. For this procedure, the selection is High Speed PCI. Cleans/Skips tab card • Number of Cleans: 1 • Number of Strips per Clean : 512 • Minimum Block Size: 2 • Number of Blocks: 5 9.
Chapter 8 Virtual Chip Mode 107 11. Click on the Load Default Values button. This enters the default ROI values. These values are: Start pixels of 1,1; End pixels based on the Chip Y and Chip X dimensions; and Groups of 1. • Region of Interest: The settings below assume a 47x47 pixel ROI (i.
108 MicroMAX System User Manual Version 6.C achieved via the Ext Sync input on the rear of the camera. Triggering from the Ext Sync input allows you to acquire a single image per TTL pulse.
Chapter 8 Virtual Chip Mode 109 Readout Time: Reported readout time that will result with the current virtual-chip parameter values. Exposure Time: Reported current exposure tim e that will result with the value entered in the Experiment Setup dialog box.
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Chapter 9 Troubleshooting Do not attach or remove any cables while the MicroMAX system is powered on. WARNING! Introduction The following issues have corresponding troubleshooting sections in this chapter.
112 MicroMAX System User Manual Version 6.C Baseline Signal Suddenly Changes If you observe a sudden change in the baselin e signal, you may have excessive humidity in the vacuum enclosure of the camera. Turn off the controller and have the cam era repumped before resuming normal operation.
Chapter 9 Troubleshooting 113 Driver=apausb.sys Port=0 ID=523459 2. Change the "Name=" entry to something more m eaningful for you (for example, ST133USB - to indicate that this is a PVCAM-based system using an ST-133 with a USB 2.0 interface) and save the edited file.
114 MicroMAX System User Manual Version 6.C 5. After inspecting and if necessary, changing th e fuses to those required by the selected voltage, reinstall the holders with the arrow facing to the right. 6. Close the power m odule and verify that the correct voltage setting is displayed.
Chapter 9 Troubleshooting 115 • For a TE-cooled camera, the camera's intern al temperature m ay be too high, such as might occur if the operating environment is particularly warm or if you are attempting to operate at a temperature colder than the specified limit.
116 MicroMAX System User Manual Version 6.C Data Overrun Due to Hardware Conflict message Figure 64. Data Overrun Du e to Hardware Conflict dialog bo x If this dialog box appears when you try to acquire a test image, acquire data, or run in focus mode, check the CCD array size and then check the DMA buffer size.
Chapter 9 Troubleshooting 117 not be suitable for the USB 2.0 interface. Therefore, we recommend replacem ent of the USB 2.0 interface module with our TAXI interface module and Princeton Instrum ents (RSPI) PCI card. If this is not the case and data o verruns continue to occur, contact Technical Support (see page 164 for contact information).
118 MicroMAX System User Manual Version 6.C 5. You should now be able to open WinView and, from Setup|Hardware…, r the Hardware Wizard. When the PVCAM dialog box ( un 6. s finished, the Use PVCAM checkbox nts and acquire data. Figure 67 ) is displayed, click in the Yes radio button, click on Next and continue through the Wizard.
Chapter 9 Troubleshooting 119 Figure 69. RSConfig dialog box: Tw o Camera Styles 5. Using Notepad or a similar text editor, open PVCAM.INI, which is located in the Windows directory (C:WINNT, for example).
120 MicroMAX System User Manual Version 6.C Detector Temperature, Acquire, and Focus are Grayed Out (Versions 2.5.19.0 and earlier) These functions and others will be deactivated if you have installed a camera being run under USB 2.0 and have opened WinV iew/32 without having first turned on the ST-133.
Chapter 9 Troubleshooting 121 Note: The [Camera_#] must be changed so the camera supported by the USB interface will be recognized (the USB driver is "apausb.sys"). For consistency, you may also want to change the camera names. 6. Save the file.
122 MicroMAX System User Manual Version 6.C will have been made by the user and the BIOS will not know which addresses and interrupts have been user assigned. When a PCI card is installed, the BIOS checks for available addresses and interrupt levels and automatically assigns them so that there are no PCI address or interrupt conflicts.
Chapter 9 Troubleshooting 123 level on the conflicting Network card in this exam ple. It is up to the user to consult the documentation for any ISA cards to determine how to make the necessary change.
124 MicroMAX System User Manual Version 6.C No CCD Named in the Hardware Wizard:CCD dialog (Versions 2.5.19.0 and earlier) Figure 73. Hardware Wizard: Detector/C amera/CCD dialog box If you have installed a USB 2.
Chapter 9 Troubleshooting 125 7. Restart the WinX application and begin acquiring data or focusing. If you see the message again, increase the DMA buffer size.
126 MicroMAX System User Manual Version 6.C packaging is usually an antistatic bag that will protect the module components from electrostatic discharge. To Install a Module: Installing a module is a bit more complex because you first have to be sure the locking screws are aligned correctly .
Chapter 9 Troubleshooting 127 Securing the Detector-Controller Cable Slide Latch Some Princeton Instruments Detector-Controlle r cables use a slide latch to secure the Detector-Controller cable to the DETECTOR connector on the back of the ST-133.
128 MicroMAX System User Manual Version 6.C Serial violations have occurred. Check interface cable. Figure 76. Serial Violati ons Have Occurred dialog box This error message dialog will appear if you tr y to acquire an image or focus the camera and either (or both) of the following conditions exists: • The cam era system is not turned ON.
Appendix A Specifications CCD Arrays Spectral Range Typically: 370-900 for MicroMAX:512BFT 350-1000 for MicroMAX:1024B and 1024BFT 400-1000 for MicroMAX:782Y and 782YHS 430-1050 for MicroMAX:512FT, 1024, and 1024FT 300-1080 for MicroMAX:1300Y, 1300YHS, and 1300YHS-DIF Types The following list is not necessarily current.
130 MicroMAX System User Manual Version 6.C Temperature Control Setting Mechanism: Temperature is set by the application software. Display: The actual tem perature can be displayed at the computer by the application software.
Appendix A Specifications 131 Shutters The round head camera does not have an internal shutter. The rectangular head camera is supplied with either a 25 mm internal shu tter (C-m ount) or a 35 mm internal shutter (F-mount). * The Shutter Compensation times listed below are based on the values used by the WinView/32 program.
132 MicroMAX System User Manual Version 6.C Programmable Interface TTL I/O at DB25 connector. Eight input bits and eight output bits are provided for control via the TTL connector. Refer to " TTL Control ", starting on page 85 , for more inform ation.
Appendix B Outline Drawings Detectors C-MOUNT (1.00-32 THREAD) 4.63 4.63 2.11 6.16 7.05 CCD at .690 OPTICAL DEPTH 0.000 MOUNTING SURF ACE COOLING AIR INLET TYPICAL BOTH SIDES COOLING AIR OUTLET TYPICAL BOTH SIDES OPTIONAL TRIPOD MOUNT KIT (2550-0312) 0.
134 MicroMAX System User Manual Version 6.C 4.63 F-MOUNT (2 3/8” - 20 THREAD NIK ON ADAPTER SHO WN 4.63 OPTIONAL TRIPOD MOUNT KIT (2550-0312) COOLING AIR OUTLET TYPICAL BOTH SIDES COOLING AIR INLET TYPICAL BOTH SIDES 8.18 0.50 0.000 CCD at 1.831 OPTICAL DEPTH NIKON F-MOUNT EXTERNAL SHUTTER JA CK 1.
Appendix B Outline Drawings 135 NOTES: 1. WEIGHT : 7.0 LBS. 2. POWER DISSIP A TION: 90 WATTS AT FULL POWER. 3. AIRFLOW : 30 CFM F AN CAP ACITY A T FULL POWER. EXTERNAL SHUTTER JACK 1.14 1.54 3.16 GAIN SWITCH ACCESS DB-25 MALE TO CONTROLLER 0.94 4.63 4.
136 MicroMAX System User Manual Version 6.C NOTES: 1. WEIGHT : 7.0 LBS. 2. POWER DISSIP A TION: 90 WATTS AT FULL POWER. 3. AIRFLOW : 30 CFM F AN CAP ACITY A T FULL POWER. EXTERNAL SHUTTER JACK 1.14 1.54 3.16 GAIN SWITCH ACCESS DB-25 MALE TO CONTROLLER 0.
Appendix B Outline Drawings 137 Note: All dimensions are in inches 1/4–20 X 3/8 MTG. (4 PLACES) A SECTION A A HOUSING CCD “C” MOUNT AD APTER W/ SHUTTER QU ARTZ WINDOW 2.49 INNER NOSE V ACUUM POR T 0.69 (FOCAL PLANE) 0.41 0.13 0.00 2.49 0.30 0.90 1.
138 MicroMAX System User Manual Version 6.C Figure 82. 1 MHz Round Head Camera: F-Mount Adapter.
Appendix B Outline Drawings 139 ST-133B Controller 13.91 5.80 8.59 Figure 83. ST-133B Controller Di mensions ST-133A Controller 5.25 13.63 8.75 Figure 84.
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Appendix C Repumping the Vacuum Introduction Round head cameras are normally shipped with a vacuum level of ~10 m Torr or better to assure proper cooling performance and to prevent condensation from collecting on the CCD. This condensation obscures or interferes with optical signals, and can leave behind harmful contaminants.
142 MicroMAX System User Manual Version 6.C Figure 85. Vacuum Connector Required for Pumping • Phillips screwdriver and a 3/16" nut driver, required to remove the back plate from the camera. Vacuum Pumpdown Procedure The instructions that follow are for a 1 MHz or 100kHz/1 MHz round head camera only.
Appendix C Repumping the Vacuum 143 Figure 87. Attaching the Vacuum Connector Figure 88. Opening the Camera to the Vacuum System.
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Appendix D Spectrometer Adapters Princeton Instruments offers a variety of sp ectrometer adapters for rectangular head (NTE) MicroMAX systems. The mounting instru ctions for these adapters are organized by spectrometer model, camera type, and adapter kit number.
146 MicroMAX System User Manual Version 6.C Acton (NTE with or without shutter) 1 Spacer Plate (remov ed) Adapter (supplied with spectrometer) Qty P/N Description 1. 3 2826-0127 Screw, 10-32 × 1/4, Button Head Allen Hex, Stainless Steel Assembly Instructions 1.
Appendix D Spectrometer Adapters 147 Chromex 250 IS (NTE with or without shutter) 3 4 5 2 1 Qty P/N Description 1. 1 2517-0901 Plate, Adapter-Female 2. 4 2826-0283 Screw, 10-32 × 3/4, Socket head, Stainless Steel, Hex, Black 3. 1 2518-0107 Adapter-Male, HR320 4.
148 MicroMAX System User Manual Version 6.C ISA HR 320 (NTE with or without shutter) 3 2 1 5 5 4 Remov e spectrometer cover f or these screws . Qty P/N Description 1. 1 2518-0106 Adapter-Female, HR320 2. 3 2826-0087 Screw, M5-10, Flat Head, Socket, Stainless Steel 3.
Appendix D Spectrometer Adapters 149 ISA HR 640 (NTE with or without shutter) 3 4 5 1 2 Qty P/N Description 1. 1 2518-0203 Adapter-Female, HR640 2. 4 2826-0144 Screw, M4-.7 × 14, Socket Head Cap, Stainless Steel 3. 1 2518-0107 Adapter-Male, HR320 4. 3 2826-0127 Screw, 10-32 × 1/4, Button Head Allen Hex, Stainless Steel 5.
150 MicroMAX System User Manual Version 6.C JY TRIAX family (NTE without shutter) 2 1 Remov e 4 screws Flanged Spectrometer Mount Qty P/N Description 1. 1 2518-1000 Adapter, TRIAX, NTE, 7377, 7376, 7413 2. 4 2826-0191 Screw, 10-32 × 5/8, Socket Head, Stainless Steel, Hex, Black Typically, the adapter is shipped already m ounted to the c a m er a.
Appendix D Spectrometer Adapters 151 SPEX 270M (NTE with or without shutter) 3 1 2 5 4 Qty P/N Description 1. 1 2518-0691 Female Adapter Plate, 2.400 ID 2. 6 2826-0068 Screw, 6-32 × 3/8, Socket Head, Cap, Stainless Steel 3. 1 2518-0690 Adapter, Focusing, Male, Spec 270 4.
152 MicroMAX System User Manual Version 6.C SPEX 500M (NTE with or without shutter) 4 1 5 2 3 Qty P/N Description 1. 1 2517-0214 Adapter-Female, Spex 500m 2. 8 2826-0170 Screw, 1/4-20 × 0.51, Low Socket Head Cap, Black 3. 1 2518-0223 Adapter-Male, Spex 500m 4.
Appendix D Spectrometer Adapters 153 SPEX TripleMate (NTE with or without shutter) 1 2 6 5 4 7 3 Qty P/N Description 1. 1 2518-0184 Adapter-Male, LN/TE, CCD/For Spex TripleMate 2. 4 2826-0128 Screw, 10-32 × 5/8, Socket Head Cap, Stainless Steel, 3. 1 2517-0163 Slit Mount, Spex 4.
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155 Appendix E USB 2.0 Limitations The following inform ation covers the curr ently know n limitations associated w ith operating under the USB 2.0 interface. • Maxim um cable leng th is 5 meters (16.4 feet) • 1 MHz is currently the upper digitization rate lim it for the ST-133 Controller.
156 MicroMAX System Us er Manual Version 6.C Feature Supported with USB 2 .0 in WinX 2.5.X Remarks Macro Record YES Macros recorded for n on- PVCAM cam eras may have to be re- recorded to functio n TT L I/O NO Table 19. Features Supported under USB 2.
Declarations of Conformity This section of the MicroMAX manual contai ns the declarations of conformity for MicroMAX sy stems. MicroMAX systems encompass RTE (round thermoelectrically- coole d) and NTE (rectangular thermoelectrically -cooled) camera heads and their associated controllers.
DECLARATION OF CONFORMITY We, ROPER SCIENTIFIC (PRINCETON INSTRUMENTS) 3660 QUAKERBRIDGE ROAD TRENTON, NJ 08619 Declare under our sole responsibility, that the product MicroMAX SYSTEM With RTE/CCD CAM.
DECLARATION OF CONFORMITY We , , ROPER SCIENTIFIC (PRINCETON INSTRUMENTS) 3660 QUAKERBRIDGE ROAD TRENTON, NJ 08619, Declare under our sole responsibility that the product ST-133 1 MHz HIGH POWER CONTR.
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Warranty & Service Limited Warranty Princeton Instruments, a division of Roper Scientific, Inc. ("Princeton Instruments", "us, "we", "our") makes the following limited warra nties.
162 MicroMAX System User Manual Version 6.C Sealed Chamber Integrity Limited 24 Month Warranty Princeton Instruments warrants the sealed chamber integrity of all our products for a period of twenty-four (24) months after shipment.
Warranty & Service 163 Owner's Manual and Troubleshooting You should read the owner’s manual thoroughl y before operating this product. In the unlikely event that you should encounter di ff.
164 MicroMAX System User Manual Version 6.C of any warranties implied by state law or non-U.S. laws, as hereby limited, the forgoing warranty is exclusive and in lie u of all other warranties, guarantees, agreements, and similar obligations of m anufacturer or seller with respect to the repair or replacement of any parts.
Index #-B C 64-pin DIN connector ...................................... 18, 126 Cables ...................................................................... 27 A/D converters ........................................................ 71 fiber optic ...
166 MicroMAX System User Manual Version 6.C C-mount ................................................................... 36 DMA buffer ........................................................... 107 assembly ............................................
Index 167 Focusing .................................................................. 51 alignment ............................................................. 54 aperture adjustment ............................................. 35 composite video output .
168 MicroMAX System User Manual Version 6.C N-O N d operation ...................................... 80 N .......................................... 94, 96, 98, 99 O O O run ................................................................ 81 PC PC Pr Pr Quan Re fer .
Index 169 Shutter (cont.) signs of failure .................................................. 128 signs of failure ..................................................... 17 ST-133 connector ................................................ 21 Shutter Power connector.
170 MicroMAX System User Manual Version 6.C Warnings (cont.) W nd troubleshooting ................. 163 software ............................................................. 162 vacuum integrity................................................ 162 XP vacuum cham ber .
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