Instruction/ maintenance manual of the product ASP300 Leica
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Instruction manual Leica ASP300 V1.3 English - 12/2003 Always keep this manual together with the instrument. Read this instruction manual carefully before working with the instrument.
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3 Leica ASP300 NOTE The information, numerical data, notes and value judgments contained in this manual rep- resent the current state of scientific knowl- edge and state-of-the-art technology.
4 Instruction manual V 1.3 – 12/2003 Table of contents 1 . Important notes .............................................................................................................. ............... 6 1.1 Symbols used in this manual .............
5 Leica ASP300 Table of contents 5.2.6 Editing the stations .................................................................................................................. 4 6 5.2.7 Reagent groups ...................................................
6 Instruction Manual V 1.3 – 12/2003 1.1 Symbols used in this manual Warnings appear in a grey box and are marked by a warning triangle . Notes, i.e. important user information appear in a grey box and are marked by an information symbol . Flammable solvents and reagents are marked with this symbol.
7 Leica ASP300 2. Safety 2. 1 Safety instructions This instruction manual includes important in- formation related to the operating safety and maintenance of the instrument and it is an im- portant part of the product.
8 Instruction manual V 1.3 – 12/2003 2. Safety 2. 2 Warnings The safety devices installed in this instrument by the manufacturer only constitute the basis for accident prevention.
9 Leica ASP300 Warnings - working with the instrument The Leica ASP300 may only be operated by trained laboratory personnel and as per the present instruction manual. In case of emergency switch off mains (item no. 5 in Fig. 3) at the rear of the instrument and unplug the power cord.
10 Instruction manual V 1.3 – 12/2003 2. 3 Integrated safety devices The instrument is equipped with the following protection features: • overpressure protection, • protection against excessive vacuum, • overcurrent protection, • overheating protection.
11 Leica ASP300 3. Instrument components and specifications 3. 1 Specific instrument options • R eagent M anagement S ystem (RMS). • The specimen cassette baskets may be used with or without cas- sette dividers and spring separators designed to separate cassettes and improve fluid penetration.
12 Instruction manual V 1.3 – 12/2003 3. 2 Overview - instrument components 3. Instrument components and specifications 2 3 4 5 13 6 7 14 15 16 17 18 19 20 21 22 12 1 1 8 10 9 1 Fig.
13 Leica ASP300 Overview - Instrument components 1 - Basic instrument - processor module 2 - Basic instrument - reagent module 3 - Wax bath lids 4 - Retort lid 5 - Touch screen 6 - Cover flap of instr.
14 Instruction manual V 1.3 – 12/2003 3. Instrument components and specifications 3. 3 Standard delivery - packing list Leica ASP300 basic standard delivery: 1 Leica ASP300 - Basic instrument 0476 34100 1 Accessory kit consisting of: 0476 35929 - 1 Basket hook 0476 34713 - 3 Specimen baskets, assy.
15 Leica ASP300 3. 4 Technical data Nominal voltage: Two factory-preset voltage settings (not user-adjustable): 100 to 120 V + 1 0% or 230 to 240 V + 1 0% Nominal frequency: 50 to 60 Hz Main fuses: 2 fast-blowing melting fuses, 20 x 5 mm, UL-listed Order no.
16 Instruction manual V 1.3 – 12/2003 Retort Capacity: max. 300 cassettes Reagent volume (in liters): 4.3 Temperature (paraffin): 40 to 65 °C Temperature (processing reagents): ambient or 35 to 55 o C Temperature (cleaning reagents): 50 to 65 °C Temperature accuracy: + 1 K Filling time: approx.
17 Leica ASP300 3. Instrument components and specifications 3.4 Technical Data (continued) Hardware and Software: • Large color liquid crystal display (LCD) and touchscreen. • User-friendly, intelligent software. • 3,5“ floppy drive and printer port.
18 Instruction manual V 1.3 – 12/2003 3. 5 Compatible reagents Fixatives 1. Formalin, buffered or unbuffered 2. Formalin saline Dehydration 1. Ethanol 4. Butanol 2. Isopropanol 5. Industrial Methylated Spirits 3. Methanol Clearing 1. Xylen (recommended) 2.
19 Leica ASP300 Moving the instrument The instrument must be installed such that the mains switch at the rear of the instrument (item no. 5 in Fig. 3) can easily be ac- cessed at any time.
20 Instruction manual V 1.3 – 12/2003 4. Setting up the instrument • Connect mains cable ( 6 ) to mains power supply socket ( 1 ) (see Fig. 3 ). • Jumper cable ( 3 ) connects the mains power supply output ( 2 ) to the electronics module input ( 4 ).
21 Leica ASP300 4. Setting up the instrument • Unpack the activated carbon filter and in- sert it into the instrument (see Fig. 4 ). Make sure to insert the filter with the correct side facing upwards. See label ( 6 ) at the front of the filter - the arrow must point upwards.
22 Instruction Manual V 1.3 – 12/2003 4. 4 Setting up the data connections Printer (optional) • Lift off the instrument console flap ( 12 ). • Connect the printer to the printer port ( 7 ) via a standard Centronics cable (refer Fig.
23 Leica ASP300 4. 5 Alarm functions The Leica ASP300 is equipped with two different alarm functions: Instrument alarm This alarm is generated from within the Leica ASP300. The instrument alarm is generated in all alarm situations. Remote alarm This alarm device is installed outside of the Leica ASP300.
24 Instruction Manual V 1.3 – 12/2003 • After switching it on the instrument will take a few minutes to initialize (see opposite - Fig. 7 ). • After initialization, the FAVORITES screen will be displayed ( Fig.
25 Leica ASP300 System diagnostics menu If, during initialization, ‘Touch here ...’ is touched (refer Fig. 7 ) and sub- sequently the supervisor pass- word is entered, the SYSTEM DIAGNOSTICS menu ( Fig. 9 ) opens up, giving access to standard in- strument settings.
26 Instruction manual V 1.3 – 12/2003 4. 7 Touch screen functions 4.7.1 User interface - overview The Leica ASP300 is programmed and operated via a colored LCD touch screen. An on-line help feature provides detailed information on all instrument functions.
27 Leica ASP300 4.7.2. Access control The Leica ASP300 may be configured to allow two levels of user ac- cess. Operator access level: • Operators may run programs and view results. On this level, the OP- ERATOR symbol is displayed in the upper right corner of the touch screen.
28 Instruction manual V 1.3 – 12/2003 4. Setting up the instrument 4.8 Check list for first operation (at supervisor access level) Parameter settings Enter instrument name and select lan- guage. Activate printer (optional). Enter carbon filter age warning and pump warning threshold.
29 Leica ASP300 5. 1 Reagent handling 5.1.1 Fill/drain reagents (other than wax) 1. Manually changing the reagents • Remove the appropriate reagent bottle from the Leica ASP300 re- agent cabinet, remove the bottle screw cap. • Drain/refill the reagent by pouring to/from a bulk container.
30 Instruction Manual V 1.3 – 12/2003 Select station Press key symbol Fig. 12 → 5. Operation Remote drain • On the screen, select reagent bottle no. 3 and the bulk container ( Remote ). The press STATION DRAIN . • Check for leaks. Remote fill • On the screen, select reagent bottle no.
31 Leica ASP300 5. Operation 5.1.2 Changing the wax Draining the wax • Connect the paraffin drain hose ( 2 ) as shown in Fig. 13 and insert the loose end of the hose into a remote collecting container. Fig. 13 • Select the wax bath to be drained and the remote container (‘Remote’).
32 Instruction Manual V 1.3 – 12/2003 Fig. 14 → → • Remove the splash protector. • Fill the wax bath ( Fig. 15 ) with wax pellets up to the top. • Press BACK and REAGENT STATUS to ac- cess the reagent status table ( Fig. 14 ). High- light the wax bath you just filled and press SET AS FULL .
33 Leica ASP300 Fig. 16 → → Adding molten paraffin • Molten wax may have a maximum temperature of 70 ° C. • If filling with molten wax, do not fill above the MAX level (see Fig. 15 ). • After a wax bath has been filled, it must be marked as ‘full’ in the REAGENT STATUS screen.
34 Instruction manual V 1.3 – 12/2003 5. 2 Setting up the instrument parameters From the start screen, press MENU to go to the MENU FUNCTIONS screen, then press MORE . In the MORE MENU FUNCTIONS screen, press SYSTEM SETUP . 5.2.1 System setup → 5.
35 Leica ASP300 Program Options Selection of the options on how programs are carried out. • STATION ALLOCATION : By age or Sequential By age - RMS is activated, reagents will automatically be used in order of increasing cleanliness. Sequential - reagents will be used in sequential order of stations.
36 Instruction manual V 1.3 – 12/2003 Security To determine the level of access to the instrument. • SUPERVISOR PASSWORD : Enabled or Disabled Enabled - A password is required for access to the supervisor level. Disabled - All instrument and software functions are fully accessible to all staff.
37 Leica ASP300 5.2.2 Editing the reagent list Adding new reagents 1. Enter the reagent name and allocate the new reagent to the reagent group it belongs to. 2. Define the stations (bottles) that will be filled with the new reagent. 3. Fill the stations (bottles) according to the reagent list.
38 Instruction manual V 1.3 – 12/2003 Allocation of reagents to the correct reagent group is the basis for compatibility monitoring. Allocation to the wrong reagent group can lead to reagent cross contamination. Entering / modifying reagent thresholds • Highlight the reagent to be modified.
39 Leica ASP300 Deleting reagents • Highlight the reagent to be deleted in the SET UP REAGENTS AND WARNING THRESHOLDS screen. • Press DELETE . • Press OK in the screen prompt. Please remember that a reagent which is already used in a pro- gram cannot be deleted.
40 Instruction manual V 1.3 – 12/2003 5. Operation 5.2.3 V ie wi ng t he pr og ra m list This list ( Fig. 20 ) displays all programs currently existing in the ASP300. You can create: • up to 15 processing programs, • 3 retort cleaning programs, • 1 wax cleaning program.
41 Leica ASP300 5. Operation 5.2.4 Adding and/or modifying programs Creating a new program • You must be logged on at supervisor level. • In the VIEW/EDIT PROGRAMS screen ( Fig. 20 ) highlight a program as similar as possible to the program you wish to create.
42 Instruction manual V 1.3 – 12/2003 5. Operation Fig. 21 Editing program steps • To edit a step, highlight the corresponding line and press the re- spective headline. • In the entry windows that pop up enter / select the program step set- tings.
43 Leica ASP300 Editing program steps (continued) Setting a delay step A so-called ‘delay step’ ensures that a program is finished at a specified time. • Highlight the program step you wish to define as a delay step. • Press DELAY . The delay symbol is moved to the selected step, thus defining the step as delay step.
44 Instruction manual V 1.3 – 12/2003 5.2.5 Favorites (maximum of 5 programs) Favorites can be programmed to end either: • “as soon as possible“ (ASAP) or • at a predetermined finish time. Adding and/or modifying Favorites • Highlight the program line to be altered.
45 Leica ASP300 Assigning a finish day • Press DAY . • Select the desired week day in the list and press OK to confirm. Assigning an icon to a “Favorite“ • Press SYMBOL in the table header. • Select the desired icon and press OK to confirm.
46 Instruction Manual V 1.3 – 12/2003 Fig. 23 ↓ → → Adding / changing reagents Highlight the desired station. • Press REAGENT NAME . • The SELECT REAGENT entry window pops up. Only those reagents that are compat- ible with the respective station will be available for selection.
47 Leica ASP300 Fig. 24 ↓ → → 5.2.7 Reagent groups Selecting a new color for a reagent group • Highlight the line containing the reagent color you wish to change. • Press COLOR in the table header: the entry window SELECT COLOR pops up. • Select the desired color and press OK to confirm.
48 Instruction Manual V 1.3 – 12/2003 Fig. 25 Fig. 26 5. 3 Programs Programs can be started from two different screens: In the Favorites screen, press ALL PROGRAMS . A ”Favorite” is a processing program that is frequently used. All settings are already programmed.
49 Leica ASP300 Fig. 28 ↓ Fig. 29 ↓ Fig. 27 5.3.1 Editing a selected program To modify a program prior to starting it, press EDIT to access the Edit Program screen. ↓ Redefine the program finish time: • Press FINISH TIME . In the entry window, set a new finish time.
5. Operation Access the RUN OPTIONS function to display the options selected for the current program. • LEVEL CHECK enabled: The lower level sensor in the retort is acti- vated.
51 Leica ASP300 • Press START to start the program. Press OK to confirm the query that follows. Enter the number of blocks, if the NUMBER OF BLOCKS op- tion has been enabled. • The FINISH TIME button shows the time when the current program will be finished.
52 Instruction Manual V 1.3 – 12/2003 → 5. 4 Reagent status In the reagent status screen ( Fig. 32 ) you can display and update the actual reagent status of each station and you can set the stations as full or empty. Fig. 32 • Warning thresholds that trigger warning messages appear on a red background.
53 Leica ASP300 5. Operation 5. 5 System Monitor The SYSTEM MONITOR screen supplies important infor- mation on the Leica ASP300 system status. After replacing the filter press RESET . ROTARY VALVE RETRIES: If a figure higher than 50 is indicated, a preventive maintenance is recommended.
54 Instruction Manual V 1.3 – 12/2003 Detailed information on how to create and install customer-specific help files can be found in the “What is Custom Help?“ help text. Accessing ‘Help’ in the event of error messages • Press DETAILS , or • Press the HELP button on the error message.
55 Leica ASP300 → → ↓ ↓ Printing run logs Printing is possible when the PRINT symbol is displayed. For the PRINT symbol to be displayed, a printer must have been configured for the instrument.
56 Instruction manual V 1.3 – 12/2003 6. 1 General The Leica ASP300 is equipped with a trouble shooting system. If an error is detected, detailed error messages and corresponding user instructions on what to do are displayed on the touch screen.
57 Leica ASP300 → 6. 3 Trouble shooting • Check for error messages indicating the nature of the problem. • Press the Help button on the message for any information relating to the cause. • Check the Run Log for any information on the nature of the failure.
58 Instruction manual V 1.3 – 12/2003 6. 4 Typical fill or drain problems Fill and drain problems may be due to several causes: 1. There is insufficient reagent • Verify that both the reagent bottles and the wax stations have sufficient reagent. 2.
59 Leica ASP300 7. Cleaning and maintenance 7. 1 Clean programs You find the clean programs at the end of the programs list ( Fig. 35 ). To display a clean program, highlight the re- spective line and press EDIT . The ASP300 offers three retort clean programs and one paraffin clean program.
60 Instruction manual V 1.3 – 12/2003 Editing steps • To edit a program step highlight the corresponding line and press the respective table header. • To disable a step, set the number of cycles to “ 0 “. Only cleaning wa- ter steps can be disabled.
61 Leica ASP300 → → 7.1.2 Wax clean programs The Leica ASP300 contains a wax clean fea- ture that removes solvents ( xylene ) from used wax. Follow the instructions on the left ( Fig. 36 ) to run a wax clean program. The duration of the wax clean program is user-programmable.
62 Instruction manual V 1.3 – 12/2003 7.1.3 Smart clean The Smart Clean prepares the ASP300 for transport. Prior to starting a Smart Clean, a complete retort clean program must be run. To start a Smart Clean, press SMART CLEAN on the MORE MENU FUNC- TIONS screen and press OK to confirm.
63 Leica ASP300 7. 2 General cleaning steps Paraffin wax baths Warning! Be careful as the walls of the wax baths are very hot and may cause burns! • Wipe the wax baths and lids clean. The lid can be removed for cleaning purposes. • If they are dirty, remove the wax strainers from the wax baths.
64 Instruction Manual V 1.3 – 12/2003 Exchanging the activated carbon filter • Exchange the filter as shown in Fig. 40 . • Replace the filter at least every four months – make sure to insert correctly – (see arrow on label)! • Perform a Smart Clean prior to changing the filter.
65 Leica ASP300 7.3.1 Daily cleaning and maintenance Cleaning the retort lid • Remove wax from the inside of the retort lid with the plastic scraper provided. Make sure to remove all wax from around the retort lid seal. • For convenience, the lid may be removed during cleaning by lifting it vertically and sliding it towards the console.
66 Instruction manual V 1.3 – 12/2003 This procedure is particularly important with bottles containing chloroform. Chloroform tends to swell the bottle seals, making them difficult to remove.
67 Leica ASP300 7. Cleaning and maintenance Maintenance activity daily w eekly monthly every 4 months Lubricate reagent bottle O-rings √√ ∗ and check for damage. Wipe dry retort and lid √ after completing retort clean cycle. Clean exterior of instrument √ with soft cloth and minimal amount of xylene.
68 Instruction manual V 1.3 – 12/2003 7. Cleaning and maintenance Maintenance activity daily w eekly monthly every 4 months Inspect and, if necessary, √ clean wax bath strainers . Inspect wax bath air vent and, √ if necessary, clear. Inspect and empty condensate bottle.
69 Leica ASP300 8. Warranty and service Warranty Leica Microsystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality con- trol procedure ba.
70 Instruction manual V 1.3 – 12/2003 Notice.
An important point after buying a device Leica ASP300 (or even before the purchase) is to read its user manual. We should do this for several simple reasons:
If you have not bought Leica ASP300 yet, this is a good time to familiarize yourself with the basic data on the product. First of all view first pages of the manual, you can find above. You should find there the most important technical data Leica ASP300 - thus you can check whether the hardware meets your expectations. When delving into next pages of the user manual, Leica ASP300 you will learn all the available features of the product, as well as information on its operation. The information that you get Leica ASP300 will certainly help you make a decision on the purchase.
If you already are a holder of Leica ASP300, but have not read the manual yet, you should do it for the reasons described above. You will learn then if you properly used the available features, and whether you have not made any mistakes, which can shorten the lifetime Leica ASP300.
However, one of the most important roles played by the user manual is to help in solving problems with Leica ASP300. Almost always you will find there Troubleshooting, which are the most frequently occurring failures and malfunctions of the device Leica ASP300 along with tips on how to solve them. Even if you fail to solve the problem, the manual will show you a further procedure – contact to the customer service center or the nearest service center
