Instruction/ maintenance manual of the product UV-1 GE
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P H A R M A C I A B I O T E C H Monitor UV -1 User Man ual Edition A G 59-7797-01.
Important user information Reading this entire manual is recommended for full understanding and use of this product. ● The exclamation mark within an equilateral triangle is intended to alert the user to the presence of important operating and maintenance instructions in the literature accompanying the instrument.
Contents 1. Int r oductio n ....................................................................................... . 4 2. General Descriptio n ........................................................................ . . 5 2. 1 Basic principl e ......
1. Introduction 1. Introduction The UV-1 is a fixed wavelength UV monitor , consisting of a control unit and an optical unit that can be positioned up to 10 meters apart. A mercury lamp is the stable light source , furthermore a built-in reference cell eliminates baseline drift.
2. General Description 2. General Description 2.1 Basic principle GE Healthcare LKB Monitor UV-1 consists of an optical unit containing the flow cell , lamp , filter assemblies and preamplifiers , and a control unit containing the signal processing circuits.
2. General Description Fig. 2 Block diagram The photocurrent from each detector is amplified in a pre-amplifier before passing to the signal processing circuitry in the control unit. If transmission is to be monitored , the signal passes directly to the low pass filter.
2. General Description 2.2 Optical unit F r ont panel cont r ols Fig. 3. Optical unit. F ront panel. No. Item Description 1 Cel l holder The complete cell holder is rem o v ed b y tu r ning the lo c king (Fig .
2. General Description Rear panel contr ols Fig. 4. Optical unit. Rear panel. No. Item Description 6 Locking knob The cell holder is in loc ked position when the loc king knob is turned fully in the direction of the arrow 7 Filter inlet Filters, con verters or aper tures are inser ted in the positions indicated.
2. General Description 2.3 Cont r ol unit F r ont panel cont r ols Fig. 5. Control unit. F ront panel. No. Item Description 12 Absorbanc e r ange Selector f or the desired absorbance r ange selector 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1 or 2 A U full scale deflection .
2. General Description Rear panel controls Fig. 6. Control unit. Rear panel. No . Item Description 17 18 19 20 21 22 Mains voltage selector Fuse holder Signal outputs Mains inlet Optical unit inlet Gr.
3. Installation 3. Installation 3.1 Site UV-1 should be installed on a stable , flat surface away from all sour- requirements ces of vibration. The atmosphere should be free of both excess humi- dity and corrosive or contaminated vapours which may form deposits on the component in the optical path.
3. Installation Mains voltage Voltage selector setting Fuse 3.4 Installation of the filter 110 V 110 250 mA 130 V 130 250 mA 220-230 V 220 125 mA 240 V 240 125 mA 4. Select the mains cable corresponding to your mains outlet. Discard unwanted mains cable immediately.
3. Installation 3.5 Installation of the flow cell Monitor U V -1 accepts flow cells for Standa r d Ch r omatograph y , FPLC and industrial applications. P r oduct Material of P ath T otal dead Illuminate d Pressure Application Code No. wetted pa r ts length v olume v olume limit area S-2 Fluoro-plasti c , 2 mm 80 µl 2 µl 0.
3. Installation 3.6 3.7 Connecting The optical unit may be placed on the bench or mounted on the optical unit laboratory scaffolding. For scaffolding mounting , mount the support rod on the optical unit. The rod may be mounted horizontally or vertically (Fig.
4. Operation 4.1 Choice of wavelength 4. Operation The UV-1 can be operated at either 2 5 4 nm , 280 nm or 40 5 nm. The choice of wavelength will depend on the spectral properties of both the eluent and the substances to be detected. Proteins and poly- pep-tides containing aromatic amino acids are usually best detected at 280 and 2 5 4 nm.
4. Operation 4.3 Conversion T% AU OD OD table T% to AU (3 mm cell) (10 mm cell) and OD 1 5 10 15 20 25 30 32 35 40 45 50 55 60 63 65 70 75 79 80 85 89 90 95 95.5 97.7 2.000 1.301 1.000 0.824 0.699 0.602 0.523 0.500 0.456 0.398 0.347 0.301 0.260 0.222 0.
4. Operation 4.6 Basic operating procedure - AU 4.7 Basic operating procedure - Transmission (%T) 4.8 Shut down 1. 2. 3. 4. 1. 2. 3. 4. 5 . 6. 7. 1. Switch AU/ %T to AU (Fig. 5 :13). Set the range selector (Fig. 5 :12) to SHORT and zero the recorder with the recorder zero control.
5. Maintenance 5. Maintenance 5.1 General To ensure trouble free running , users are advised to observe the follo- precautions wing precautions: All liquids passing through the flow cell should be free of suspended particles. All liquids should be degassed to prevent air bubble formation in the flow cell.
5. Maintenance Cleaning with detergent: ● 1. Remove the cell holder from the optical unit. 2. Pump undiluted cleaning detergent through the cell for at least 2 hours. 3. Rinse the cell with a) distilled water (100 ml) b) ethanol/distilled water ( 5 0% v/v , 100 ml) c) distilled water (100 ml) 4.
8. Insert the new cell, being careful not to touch either of the optical surfaces. See that the inlet and outlet ports are corr ectly aligned. 9. Retighten the screw washers with the tool pr ovided. Do not use excessive force. 10. Check for leakage. 1 1.
4. Set the range selector to SHORT and zer o the recor der . 5 . Set the range selector as follows: 2 5 4 nm , range 1 280 nm , range 0.2 6. T urn Zer o fully clockwise. Response 8 mV or greater: the lamp is operating properly and the optical system is clean.
5. Maintenance 5.9 6 . Use an Allen key (2. 5 mm) to unscrew the upper of the two screws securing the lamp holder and the lamp. Note carefully the positions of the insulation sleeve and washer.
6. Trouble-shooting 6. Trouble-shooting The UV-1 Monitor has been designed for trouble-free use. If good chromatographic practice is followed , very little difficulty should be experienced. Clean optical surfaces are essential if low noise levels are to be maintained.
7. Technical Specifications 7. Technical Specifications Wavelength range Lamp Filters Operating modes Full scale ranges Noise Linearity Temperature drift Long term drift Time constant Recorder output .
8. Accessories and Spare Parts 8. Accessories and Spare Parts Please order accessories and spare parts according to the designation and code numbers given below.
Printed in Sweden by SNITS & DESIGN AB/Västra Aros Tryckeri, Nov 1996.
An important point after buying a device GE UV-1 (or even before the purchase) is to read its user manual. We should do this for several simple reasons:
If you have not bought GE UV-1 yet, this is a good time to familiarize yourself with the basic data on the product. First of all view first pages of the manual, you can find above. You should find there the most important technical data GE UV-1 - thus you can check whether the hardware meets your expectations. When delving into next pages of the user manual, GE UV-1 you will learn all the available features of the product, as well as information on its operation. The information that you get GE UV-1 will certainly help you make a decision on the purchase.
If you already are a holder of GE UV-1, but have not read the manual yet, you should do it for the reasons described above. You will learn then if you properly used the available features, and whether you have not made any mistakes, which can shorten the lifetime GE UV-1.
However, one of the most important roles played by the user manual is to help in solving problems with GE UV-1. Almost always you will find there Troubleshooting, which are the most frequently occurring failures and malfunctions of the device GE UV-1 along with tips on how to solve them. Even if you fail to solve the problem, the manual will show you a further procedure – contact to the customer service center or the nearest service center
